Related Experiment Videos
Isolation and characterization of complement receptor type 1 from rat glomerular epithelial cells
R J Quigg1, M L Galishoff, A E Sneed
1Department of Internal Medicine, Medical College of Virginia, Richmond.
Kidney International
|March 1, 1993
Summary
Complement receptor type 1 (CR1) is confirmed in rat glomerular epithelial cells and glomeruli. This finding establishes CR1 presence in rat kidneys, similar to humans, with potential implications for kidney research.
Area of Science:
- Nephrology
- Immunology
- Cell Biology
Background:
- Complement receptor type 1 (CR1) is found in human glomerular epithelial cells (GEC).
- CR1 presence in rat glomeruli was previously undocumented.
- Cultured rat GEC exhibit CR1-like activity with complement-coated erythrocytes.
Purpose of the Study:
- To confirm the presence of CR1 in cultured rat GEC.
- To determine if CR1 is present in rat glomeruli.
- To identify potential CR1-related proteins in rat GEC and glomeruli.
Main Methods:
- C3b affinity chromatography to isolate proteins from cultured rat GEC.
- Western blotting using anti-human CR1 antibodies.
- C3b binding assays on GEC monolayers.
- Western blotting on isolated rat glomeruli.
Main Results:
- A 200 kDa protein reactive with anti-human CR1 was isolated from cultured rat GEC.
- C3b binding to rat GEC showed low affinity and approximately 6700 binding sites per cell.
- Western blotting confirmed CR1 presence in rat glomeruli.
- A 70 kDa protein, likely Crry, was identified in both cultured GEC and glomeruli.
Conclusions:
- CR1 is present in cultured rat GEC and rat glomeruli.
- The identified 70 kDa protein is likely Crry, a CR1 homolog.
- Crry may function in rat GEC similarly to decay accelerating factor in human GEC.