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Cyclins D1 and D2 are differentially expressed in human B-lymphoid cell lines
I Palmero1, A Holder, A J Sinclair
1Imperial Cancer Research Fund Laboratories, London, UK.
Oncogene
|April 1, 1993
Summary
Cyclin D2, a protein similar to cyclin D1, is expressed in certain lymphoid tumors and cell lines. Its expression is linked to Epstein-Barr virus immortalization, suggesting distinct roles for D-type cyclins in lymphocyte development.
Area of Science:
- Molecular Biology
- Oncology
- Immunology
Background:
- Cyclin D1 is transcriptionally activated in lymphoid tumors but typically silent in lymphocytes.
- Chromosomal rearrangements can activate cyclin D1 expression in lymphoid malignancies.
Purpose of the Study:
- To identify and characterize cyclin D2 in human lymphoid cells.
- To investigate the expression patterns of cyclin D2 in relation to cyclin D1 and Epstein-Barr virus (EBV).
Main Methods:
- Isolation of cyclin D1-related complementary DNAs (cDNAs) from a B-lymphoid cell line.
- Analysis of cyclin D2 transcript levels in various lymphoid cell lines and tumor subtypes.
- Comparison of cyclin D2 expression in EBV-immortalized cells versus non-immortalized cells.
Main Results:
- Human cyclin D2 cDNA was identified, encoding a protein with 63% identity to cyclin D1.
- Cyclin D2 transcripts were detected in numerous lymphoid cell lines but not ubiquitously.
- High cyclin D2 expression correlated with EBV immortalization in specific B-cell leukemia and Burkitt's lymphoma (BL) subtypes (group III BL).
Conclusions:
- D-type cyclins may possess distinct functions during lymphocyte differentiation.
- Epstein-Barr virus (EBV) infection appears to influence cyclin D2 expression in lymphoid cells, potentially impacting B-cell lymphomagenesis.