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[The amine-forming ability of Aeromonas spp]
1Humboldt-Universität zu Berlin, Fachbereich Veterinärmedizin, Institut für Lebensmittelhygiene, Deutschland.
Abstract:
Motile Aeromonas (A.) species are considered international more and more as potential food poisoning organisms. Their ability to produce biogenic amines, products of metabolism, which in case can cause a disease, was only searched insufficiently till now. 50 strains of the species A.hydrophila, A.sobria, A.caviae and the non-motile species A.salmonicida were included in the tests for amine producing potency. Qualitative investigations to the formation of histamine, tryptamine, and tyramine were ensued by the help of thin layer chromatography. Quantitative investigations were only done in respect of the production of tyramine, which had been proved qualitatively in several strains, while histamine and tryptamine were not produced. Concentrations of tyramine with foodhygienic relevance were found out partially.
Insights
Motile Aeromonas species can cause food poisoning by producing biogenic amines. This study found that some Aeromonas strains produce relevant levels of tyramine, a potentially harmful food contaminant.
Area of Science:
- Microbiology
- Food Safety
- Biochemistry
Context:
- Motile Aeromonas species are increasingly recognized as significant foodborne pathogens.
- The role of biogenic amines, metabolic byproducts of bacteria, in causing foodborne illnesses is a growing concern.
- Previous research on the biogenic amine production by Aeromonas species is limited.
Purpose:
- To investigate the biogenic amine-producing potential of various Aeromonas species.
- To identify the specific biogenic amines (histamine, tryptamine, tyramine) produced by these strains.
- To quantify tyramine production and assess its food-hygienic relevance.
Summary:
- Fifty strains of Aeromonas hydrophila, Aeromonas sobria, Aeromonas caviae, and Aeromonas salmonicida were tested for biogenic amine production.
- Thin-layer chromatography was used for qualitative detection of histamine, tryptamine, and tyramine.
- Histamine and tryptamine were not detected; however, tyramine was qualitatively identified in several strains and quantitatively analyzed, with some strains producing food-hygienic relevant concentrations.
Impact:
- This study highlights the potential of certain Aeromonas strains to produce tyramine, a biogenic amine of food-hygienic concern.
- Findings contribute to understanding the pathogenicity of Aeromonas species and inform food safety risk assessments.
- Identifies a specific metabolic pathway (tyramine production) relevant to Aeromonas-associated foodborne illnesses.