Related Experiment Videos
Development of immortalized human articular cartilage cell lines
G Verbruggen1, A M Malfait, K F Almqvist
1Dept. of Rheumatology, Ghent University Hospital, Belgium.
Agents and Actions. Supplements
|January 1, 1993
Abstract:
Human articular cartilage cells were transfected with the t.-sensitive polyomavirus large T antigen of SV40. Several immortalized chondrocyte cell lines were obtained. The types of acidic polysaccharides and of collagen synthesized suggest dedifferentiation in the in vitro culture system used afterwards to obtain large numbers of cells.