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A radiometric microbiologic method for vitamin B12 assay

M F Chen, P A McIntyre, H N Wagner

    Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
    |April 1, 1977
    PubMed
    Summary
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    A new radiometric method accurately measures vitamin B12 in human serum using Lactobacillus leichmannii and 14C-arginine. This technique simplifies vitamin B12 assays by reducing necessary steps for improved efficiency.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate vitamin B12 quantification is crucial for diagnosing deficiencies.
    • Standard microbiologic assays for vitamin B12 can be labor-intensive and time-consuming.
    • Existing methods often require extensive sample preparation steps.

    Purpose of the Study:

    • To develop a novel radiometric microbiologic assay for vitamin B12 in human serum.
    • To optimize the assay for sensitivity and efficiency.
    • To compare the new method with the standard turbidimetric microbiologic assay.

    Main Methods:

    • Utilized Lactobacillus leichmannii and 14C-arginine to detect vitamin B12.
    • Optimized CO2 evolution using guanido-labeled arginine.

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  • Simplified protein extraction and separation steps through autoclaving.

    • Main Results:

    • The radiometric method demonstrated significant 14CO2 production proportional to cyanocobalamin levels.
    • Assay achieved satisfactory recovery of added vitamin B12 to serum samples.
    • Results were comparable to the standard turbidimetric microbiologic method.

    Conclusions:

    • The developed radiometric microbiologic method offers a simplified and efficient alternative for vitamin B12 serum analysis.
    • The method eliminates several steps from traditional assays, reducing processing time.
    • This technique provides reliable vitamin B12 measurements comparable to established methods.