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Related Experiment Videos

Developmental changes in renin gene expression in ovine kidney cortex

G M Carbone1, A U Sheikh, S Rogers

  • 1Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157.

The American Journal of Physiology
|March 1, 1993
PubMed
Summary

Renin mRNA and renin content in sheep kidneys increase during fetal development and in newborns, then decrease in adults. This suggests developmental regulation of the renin gene at the transcriptional level.

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Area of Science:

  • Renal physiology
  • Developmental biology
  • Molecular endocrinology

Background:

  • The renin-angiotensin system plays a crucial role in blood pressure regulation.
  • Understanding the developmental regulation of renin is essential for comprehending renal ontogeny.

Purpose of the Study:

  • To investigate the ontogeny of renin mRNA and active renin content in ovine kidneys.
  • To determine if renin gene expression is developmentally regulated in sheep.

Main Methods:

  • Studied ovine fetuses at different gestational ages, newborn lambs, and adult sheep.
  • Quantified renal renin mRNA using hybridization with a labeled renin cDNA probe.
  • Measured active renal renin content by quantifying angiotensin I generation.

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Main Results:

  • Renin mRNA levels and active renin content significantly increased from early to late gestation and in newborns, declining in adults.
  • A strong positive correlation was observed between renin mRNA levels and active renin content (R = 0.88).
  • Significant age-related effects were found for both renin mRNA (P < 0.001) and renin content (P < 0.003).

Conclusions:

  • The renin gene is developmentally regulated in the ovine kidney.
  • Renal active renin content is, at least partly, regulated by transcriptional events during development.