Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The hyaluronate synthase from a eukaryotic cell line

L Klewes1, E A Turley, P Prehm

  • 1Institute für Physiologische Chemie und Pathobiochemie, Münster, Germany.

The Biochemical Journal
|March 15, 1993
PubMed
Summary

Researchers identified the hyaluronate synthase complex in B6 cell plasma membranes. This complex contains two subunits responsible for hyaluronate synthesis and precursor binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Receptor for hyaluronan mediated motility (RHAMM/HMMR) is a novel target for promoting subcutaneous adipogenesis.

Integrative biology : quantitative biosciences from nano to macro·2017
Same author

Hyaluronan metabolism in remodeling extracellular matrix: probes for imaging and therapy of breast cancer.

Integrative biology : quantitative biosciences from nano to macro·2011
Same author

The expression pattern of hyaluronan synthase during human tooth development.

Archives of oral biology·2005
Same author

CD44H and the isoforms CD44v5 and CD44v6 in the synovial fluid of the osteoarthritic human knee joint.

Osteoarthritis and cartilage·2003
Same author

Peptides that mimic glycosaminoglycans: high-affinity ligands for a hyaluronan binding domain.

Chemistry & biology·2001
Same author

Active erk regulates microtubule stability in H-ras-transformed cells.

Neoplasia (New York, N.Y.)·2001

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Hyaluronate (HA) is a crucial glycosaminoglycan involved in various biological processes.
  • Understanding the enzymatic machinery responsible for HA synthesis is vital for cellular and developmental biology.

Purpose of the Study:

  • To identify and characterize the hyaluronate synthase complex in B6 cell plasma membranes.
  • To elucidate the subunit composition and functions within the complex.

Main Methods:

  • Digitonin solubilization of plasma membranes.
  • Triton X-114 phase separation for protein partitioning.
  • Immunological cross-reactivity assays using specific antibodies.
  • Affinity chromatography for protein purification.

Main Results:

  • The hyaluronate synthase complex consists of 52 kDa and 60 kDa subunits.
  • Both subunits bind the UDP-GlcA precursor and associate with newly synthesized hyaluronate.
  • The 52 kDa subunit shows homology to streptococcal hyaluronate synthase, while the 60 kDa subunit interacts with a hyaluronate receptor.
  • The 52 kDa subunit was purified to homogeneity.

Conclusions:

  • The identified 52 kDa and 60 kDa proteins constitute the functional hyaluronate synthase complex in B6 cells.
  • This complex plays a key role in hyaluronate biosynthesis and potentially its cellular interactions.

Related Experiment Videos