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A new 'solid phase' procedure to synthesize immunotoxins (antibody-ribosome inactivating protein conjugates)
1Istituto di Chimica Farmaceutica Applicata, Torino, Italy.
Farmaco (Societa Chimica Italiana : 1989)
|January 1, 1993
Abstract:
A method to produce immunotoxins (conjugates comprising of a monoclonal antibody and toxin) using ribosome inactivating protein anchored on an affinity gel derivatized with triazinic dye is described. The adsorbed toxins were activated with 2-imino-thiolane and then conjugated to monoclonal antibody activated by SPDP. The "heterogeneous phase" system offered several advantages, reducing the usually required purification steps and opening a way to automatize the conjugation procedure.