Ontogenetic study of early brain stem projections to the spinal cord in the rat

In an attempts to describe the early development of the brain stem-spinal projections, we implanted DiI crystals at the C3 level of the spinal cord of 13- and 14-day fixed embryos. After a diffusion period of 2 to 4 months, neurons of the rhombencephalic reticular formation were retrogradely labeled by the tracer. This group of neurons was situated ventromedially in the tegmentum. Their axons coursed into the ventral marginal layer at bulbar levels and entered the ventral funiculus when reaching the spinal cord. Neurons of the lateral vestibular nucleus were also labeled and gave rise to descending fibers that gradually moved medially and entered the spinal cord in the ventral funiculus. In the mesencephalon, labeled cell bodies of the interstitial nucleus of Cajal (InC) were found lying ventrally in the tegmentum, at the rostral end of the medial longitudinal fasciculus (mlf), in which their axons coursed. Also, in the midbrain, several cells lying dorsal to the InC, with axons descending in the lateral tegmentum, were tentatively identified as part of the mesencephalic reticular formation.