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Related Experiment Videos

Receptor editing in self-reactive bone marrow B cells

S L Tiegs1, D M Russell, D Nemazee

  • 1Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.

The Journal of Experimental Medicine
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

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Newly generated B cells encountering self-antigens in bone marrow can alter their antigen receptor specificity. This process, called receptor editing, involves expressing V(D)J recombinase activator genes to modify receptors.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Clonal selection is a central immunology paradigm where lymphocytes are selected by antigen.
  • Developing B cells express surface immunoglobulin M (IgM) and idiotype.
  • Studying transgenic mice allows analysis of homogeneous B cell clones.

Purpose of the Study:

  • To investigate the outcome of autoantigen encounter in developing B cells.
  • To determine if B cells can alter antigen receptor specificity in response to self-antigens.
  • To elucidate the mechanism of antigen-directed receptor modification.

Main Methods:

  • Analysis of B cells in mice transgenic for anti-H-2Kk,b antibody genes.
  • Detection of surface IgM and idiotype on immature B cells.

Related Experiment Videos

  • Assessment of V(D)J recombinase activator gene expression.
  • Examination of endogenous immunoglobulin light chain gene assembly.

    • Main Results:

    • Immature B cells binding to self-antigens (Kb or Kk proteins) in bone marrow altered their antigen receptor specificity.
    • This alteration involved the expression of V(D)J recombinase activator genes.
    • Endogenously encoded immunoglobulin light chain variable genes were assembled.

    Conclusions:

    • Self-antigen encounter can induce changes in the specificity of developing B cell antigen receptors.
    • Receptor editing is an antigen-directed mechanism for modifying lymphocyte specificity.
    • This process contributes to the generation of a diverse and self-tolerant lymphocyte repertoire.