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Persistent decrease in spermidine/spermine N1-acetyltransferase activity in 'post-regeneration' rat liver

M E Ferioli1

  • 1Centro di Studio sulla Patologia Cellulare, C.N.R., Università di Milano, Italy.

Biochimica Et Biophysica Acta
|March 21, 1993
PubMed
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Polyamines and related enzyme activity in rat livers were studied after partial hepatectomy. Enzyme activity and polyamine levels initially increased then decreased as liver mass regenerated.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Regenerative Medicine

Background:

  • Polyamines are essential for cell growth and proliferation.
  • Hepatectomy triggers liver regeneration, a complex process involving significant metabolic changes.
  • The role of polyamine metabolism, specifically spermidine/spermine N1-acetyltransferase (SSAT), during liver regeneration is not fully understood.

Purpose of the Study:

  • To investigate the dynamic changes in cytosolic spermidine/spermine N1-acetyltransferase (SSAT) activity and polyamine levels in rat livers following partial hepatectomy.
  • To correlate these changes with the process of liver mass regeneration.

Main Methods:

  • Partial hepatectomy was performed on rats.
  • Liver samples were collected at various time points post-operation.

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  • Cytosolic SSAT activity was measured.
  • Levels of putrescine, spermidine, and spermine were quantified using biochemical assays.

    • Main Results:

    • SSAT activity showed an early increase within hours after partial hepatectomy.
    • This enzyme activity significantly decreased by day 10, coinciding with liver mass regain, and remained low up to 30 days post-operation.
    • N1-acetylspermidine levels increased, and putrescine content dramatically rose, confirming early SSAT induction.
    • Spermidine levels increased during the first 10 days and then decreased, while spermine levels showed similar but less pronounced changes.

    Conclusions:

    • Early induction of SSAT activity and altered polyamine profiles accompany the initial stages of liver regeneration after partial hepatectomy.
    • The decrease in SSAT activity and normalization of polyamine levels correlate with the completion of liver mass regeneration.
    • These findings highlight the dynamic role of polyamine metabolism in regulating liver repair and regrowth.