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Identification and characterization of the major carbohydrate-binding proteins in chicken serum as immunoglobulins
1Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
The Journal of Veterinary Medical Science
|February 1, 1993
Abstract:
To isolate the major carbohydrate-binding proteins in pooled normal chicken sera, affinity chromatographies on different affinity gels were carried out. From the results obtained, normal chicken sera were found to contain serum proteins which were Ca(2+)-dependently and -independently reactive with mannose, N-acetylglucosamine, N-acetylgalactosamine, L-fucose, melibiose, lactose, unsubstituted Sepharose 4B, and SeaKem HE agarose. They were electrophoretically and antigenically found to be mainly IgM and IgG.