[Thin layer chromatography of riboflavin in urine (author's transl)]

A method is described for determining riboflavin excretion in urine by thin-Layer chromatography. The sample will be preliminarily purified on Permutit T with subsequent separation of riboflavin by thin-layer chromatography on Kieselgel HR with pyridine--acetic acid--water as an eluant. Substances interfering with the fluorescence of riboflavin can be eliminated through the procedure. Riboflavin remission is measured by a chromatogram-spectralphotometer with fluorescence equipment. Between 0.02 to 0.5 mug riboflavin intensity of fluorescence is proportional to the concentration of riboflavin.