Isolation of Antho-RFamide related peptides from the eyestalks of blue crab

1. Two carboxyl-terminally amidated peptides (CP1 and CP2) were isolated from the blue crab (Callinectes sapidus) eyestalks by a method of carboxyl-terminal analysis. 2. The peptides were sequenced as pGlu-Gly-Arg-Phe-amide (CP1) and pGlu-Leu-Gly-Arg-Phe-amide (CP2). 3. Each carboxyl-terminus of the peptides was precisely determined by amino acid analysis utilizing phenylisothiocyanate derivatives. 4. CP1 was identical to the sea anemone neuropeptide, Antho-RFamide.