Regulation of c-fos proto-oncogene expression by growth hormone: protein synthesis is not required for down-regulation
S Barcellini-Couget1, A Doglio, C Dani
1Centre de Biochimie (UMR 134 CNRS), Université de Nice-Sophia Antipolis, UFR Sciences, France.
Abstract:
Growth hormone (GH) has been previously shown in Ob1771 adipose cells to transiently stimulate the expression of the c-fos gene by a protein kinase C (PKC)-dependent pathway. This regulation takes place at a transcriptional level. In the presence of cycloheximide (CHX), stimulation by PKC activators or by serum leads to a "superinduction" of the c-fos gene. In contrast, upon GH stimulation in the presence of CHX, no superinduction takes place and neither prolonged transcription nor mRNA stabilization are observed.
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