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Purification and partial characterisation of NADP(+)-linked isocitrate dehydrogenase from rat liver cytosol
H Fatania1, K E al-Nassar, V Sidhan
1Department of Biochemistry, Faculty of Medicine, Kuwait University.
FEBS Letters
|March 29, 1993
Abstract:
NADP(+)-linked isocitrate dehydrogenase from rat liver cytosol was purified (approximately 135-fold) to apparent homogeneity in 27% yield. The purified enzyme has specific activity of 73 units.mg-1. The native enzyme showed an apparent M(r) of 94,000 by gel filtration and was composed of two identical subunits of M(r) 45,000 as judged by SDS/PAGE. In isoelectric focusing, a pI value of 5.7 was estimated for the enzyme.