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Measurement of non-enzymic glycosylation with a radiochemical assay
1Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, U.K.
The International Journal of Biochemistry
|March 1, 1993
Abstract:
1. A radiochemical assay has been developed to measure non-enzymic glycosylation (glycation), which is free from interference from enzymic glycosylation and which can be used in batch analysis. 2. Glycated residues were isolated by affinity chromatography on mini-columns, after reduction with Na[3H]BH4 and hydrolysis. 3. The specific activity of the labelled borohydride was measured by a novel procedure. 4. The method was used to measure glycation of albumin, immunoglobulin G and fibrinogen isolated from diabetic and non-diabetic subjects.