Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

K(+)-dependent Na+/Ca2+ exchange in human platelets

M Kimura1, A Aviv, J P Reeves

  • 1Hypertension Research Center, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07103.

The Journal of Biological Chemistry
|April 5, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paternal Age and Transgenerational Telomere Length Maintenance: A Simulation Model.

Scientific reports·2019
Same author

Water permeability of phospholipid vesicles.

The Journal of membrane biology·2013
Same author

Primary diffuse large B-cell lymphoma of the breast: looking at pathogenesis, clinical issues and therapeutic options.

Annals of oncology : official journal of the European Society for Medical Oncology·2013
Same author

Acute neuroleptic induced leukopenia in Ethiopian jews with benign familial leukopenia.

The International journal of risk & safety in medicine·2013
Same author

Longitudinal versus cross-sectional evaluations of leukocyte telomere length dynamics: age-dependent telomere shortening is the rule.

The journals of gerontology. Series A, Biological sciences and medical sciences·2011
Same author

Merkel cell carcinoma, chronic lymphocytic leukemia and other lymphoproliferative disorders: an old bond with possible new viral ties.

Annals of oncology : official journal of the European Society for Medical Oncology·2010
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
See all related articles

Human platelets exhibit sodium-calcium (Na+/Ca2+) exchange activity, which is dependent on extracellular potassium (K+). This study reveals a unique Na+/Ca2+-K+ exchanger in platelets, distinct from cardiac exchangers.

Area of Science:

  • Biochemistry
  • Cell Physiology
  • Molecular Transport

Background:

  • Human platelets possess a Na+/Ca2+ exchanger critical for calcium homeostasis.
  • Understanding the specific characteristics of this exchanger is important for platelet function research.

Purpose of the Study:

  • To investigate the properties of Ca2+ influx via Na+/Ca2+ exchange in human platelets.
  • To determine the role of extracellular ions, particularly potassium (K+), in Na+/Ca2+ exchange.
  • To characterize the exchanger's ion transport stoichiometry and compare it to other known exchangers.

Main Methods:

  • Fura-2 fluorescence techniques were used to measure intracellular Ca2+ levels.
  • Radiotracer flux studies with 45Ca2+ and 86Rb+ were employed to quantify ion transport.

Related Experiment Videos

  • Platelets were pretreated with ouabain to enhance internal Na+ concentration.

    • Main Results:

    • Ca2+ influx via Na+/Ca2+ exchange in human platelets was dependent on extracellular K+.
    • Potassium (K+) stimulated Ca2+ influx, with a Km of approximately 1 mM.
    • Extracellular Ca2+ stimulated 86Rb+ influx, indicating potential co-transport of Rb+ and Ca2+.
    • The observed ion transport properties suggest a Na+/Ca2+-K+ exchanger distinct from the cardiac type.

    Conclusions:

    • Human platelets possess a Na+/Ca2+-K+ exchanger with unique properties.
    • This platelet exchanger differs from the cardiac Na+/Ca2+ exchanger.
    • The findings contribute to understanding ion transport mechanisms in non-excitable cells.