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Imaging frozen, hydrated acrosomal bundle from Limulus sperm at 7 A resolution with a 400 kV electron cryomicroscope
M F Schmid1, J Jakana, P Matsudaira
1Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Journal of Molecular Biology
|March 20, 1993
Abstract:
Actin and scruin are present in a highly ordered array in the acrosomal bundle from Limulus sperm. Frozen, hydrated acrosomal bundle can be imaged in a 400 kV electron cryomicroscope using the spot-scan technique. Quantitative analysis of the h0l projection images shows diffraction data beyond 7 A.