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Large scale, liquid phase synthesis of oligonucleotides by the phosphoramidite approach
G M Bonora1, G Biancotto, M Maffini
1Department of Organic Chemistry, CNR, University of Padova, Italy.
Nucleic Acids Research
|March 11, 1993
Abstract:
A new method for the liquid phase synthesis of oligonucleotides is described which makes use of polyethylene glycol (PEG) as soluble support and phosphoramidite derivatives as synthons. The new synthetic protocol was applied to a quite large scale production (about 100 mumoles) of such compounds up to the 20mer level. This solution method, called HELP High Efficiency Liquid Phase) Plus, appears effective in terms of speed and coupling yield and can be evaluated for the production of large amount of oligonucleotides.