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Related Experiment Videos

Splicing choice from ten variant exons establishes CD44 variability

C Tölg1, M Hofmann, P Herrlich

  • 1Kernforschungszentrum Karlsruhe, Institut für Genetik, Germany.

Nucleic Acids Research
|March 11, 1993
PubMed
Summary
This summary is machine-generated.

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The CD44 protein exhibits significant diversity due to alternative splicing of its extracellular region. This process generates numerous CD44 isoforms, impacting various tissues and tumor cells, suggesting broad physiological roles.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The CD44 protein is a cell surface glycoprotein known for its significant heterogeneity.
  • This heterogeneity is attributed to posttranslational modifications and differential splicing of its gene.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying CD44 heterogeneity, specifically focusing on alternative splicing.
  • To identify the number of exons involved in the variable region of CD44 and how their combinations contribute to isoform diversity.

Main Methods:

  • Comparative analysis of complementary DNA (cDNA) clones with varying inserts in the CD44 extracellular region.
  • Sequence comparison with genomic clones from mouse models.

Main Results:

Related Experiment Videos

  • Identification of at least ten distinct exons within the variable region of the CD44 gene.
  • Demonstration that alternative splicing combines these exons in various ways to produce heterogeneous CD44 molecules.
  • Observed differential expression of CD44 isoforms in various tissues and progressing tumor cells.

Conclusions:

  • Alternative splicing is a major driver of CD44 protein heterogeneity.
  • The combinatorial usage of CD44 exons generates a wide array of isoforms.
  • These diverse CD44 isoforms likely play crucial, yet uncharacterized, physiological roles in different cell types and disease states.