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Updated: Aug 8, 2026

Analysis of Epididymal Protein Synthesis and Secretion
Published on: August 25, 2018
Ultrastructural and immunocytochemical study of P1 protamine localization in human testis
D Lescoat1, Y Blanchard, M T Lavault
1Laboratoire d'Histologie et d'Embryologie, Rennes, France.
Abstract:
Two monoclonal antibodies (MAbs) directed against human protamine P1 were realized. Anti-P1 specificity was assessed by western-blot and confirmed by ELISA. Monoclonal antibody 97-3 was selected. Protamine P1 was specifically demonstrated in human testis by immunoelectron microscopy, using 97-3 MAb and an indirect post-embedding immunogold technique. Our results clearly demonstrated the precise time of appearance of P1 protamine in the nuclei of human spermatids. P1 first appeared in the nucleus of step 5 spermatids and its concentration was increased in steps 6-8 spermatids, cytoplasm was not labelled.
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