Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzymatic basis for altered ascorbic acid and dehydroascorbic acid levels in diabetes

A M Bode1, C R Yavarow, D A Fry

  • 1University of North Dakota School of Medicine, Physiology Department, Grand Forks 58202.

Biochemical and Biophysical Research Communications
|March 31, 1993
PubMed
Summary

Diabetes alters ascorbic acid (AA) and dehydroascorbic acid (DHAA) levels due to impaired recycling. This study shows reduced AA production from DHAA in diabetic liver, linked to decreased glucose-6-phosphate dehydrogenase (G6PDH) activity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using search engine data to gauge public interest in mental health, politics and violence in the context of mass shootings.

PloS one·2020
Same author

Epstein-Barr virus encoded latent membrane protein 1 suppresses necroptosis through targeting RIPK1/3 ubiquitination.

Cell death & disease·2018
Same author

A vicious loop of fatty acid-binding protein 4 and DNA methyltransferase 1 promotes acute myeloid leukemia and acts as a therapeutic target.

Leukemia·2017
Same author

Phosphorylation of NFAT3 by CDK3 induces cell transformation and promotes tumor growth in skin cancer.

Oncogene·2016
Same author

Fatty acid-binding protein FABP4 mechanistically links obesity with aggressive AML by enhancing aberrant DNA methylation in AML cells.

Leukemia·2016
Same author

Fyn is a redox sensor involved in solar ultraviolet light-induced signal transduction in skin carcinogenesis.

Oncogene·2015

Area of Science:

  • Biochemistry
  • Metabolic Disorders
  • Cellular Redox Homeostasis

Background:

  • Abnormal plasma ascorbic acid (AA) and dehydroascorbic acid (DHAA) levels are noted in diabetes.
  • These alterations may stem from a deficiency in AA recycling pathways.

Purpose of the Study:

  • To investigate the relationship between AA/DHAA levels and the hexose monophosphate (HMP) shunt in diabetic liver.
  • To determine the role of glucose-6-phosphate dehydrogenase (G6PDH) in AA/DHAA metabolism in diabetes.

Main Methods:

  • Assaying ascorbic acid (AA) production directly and via the HMP shunt pathway.
  • Measuring dehydroascorbic acid (DHAA) reduction coupled to NADPH and glutathione reductase.
  • Quantifying glucose-6-phosphate dehydrogenase (G6PDH) activity in diabetic liver tissue.

Related Experiment Videos

Main Results:

  • Ascorbic acid (AA) and dehydroascorbic acid (DHAA) levels are altered in the diabetic liver.
  • AA production from DHAA via the HMP shunt pathway is significantly decreased in diabetic liver.
  • Glucose-6-phosphate dehydrogenase (G6PDH) activity is decreased in diabetic liver, impacting NADPH availability.

Conclusions:

  • The impaired AA/DHAA recycling in diabetes is linked to reduced activity of the HMP shunt pathway.
  • Decreased G6PDH activity in diabetic liver compromises NADPH production, essential for DHAA reduction.
  • These findings highlight a biochemical basis for altered vitamin C metabolism in diabetic conditions.