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The mouse low density lipoprotein receptor gene: cDNA sequence and exon-intron structure
M J Hoffer1, M M van Eck, F Petrij
1MGC-Department of Human Genetics, Leiden University, The Netherlands.
Biochemical and Biophysical Research Communications
|March 31, 1993
Summary
Researchers have sequenced the mouse low density lipoprotein (LDL) receptor gene, revealing its structure and homology to the human gene. This cloning advances mouse models for studying cholesterol metabolism and gene targeting.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The low density lipoprotein (LDL) receptor is crucial for cholesterol metabolism.
- Understanding the mouse LDL receptor gene aids in studying human cholesterol disorders.
Purpose of the Study:
- To determine the cDNA sequence and exon-intron structure of the mouse low density lipoprotein receptor (Ldlr) gene.
- To compare the mouse Ldlr gene with its human counterpart.
- To facilitate the use of mouse models in cholesterol metabolism research.
Main Methods:
- cDNA sequencing
- Northern blot analysis
- Gene structure determination (exon-intron mapping)
Main Results:
- The mouse Ldlr gene cDNA sequence was determined, showing 76% homology to the human gene.
- The gene comprises 18 exons, spanning 28 kb, with homologous promoter regions to the human gene.
- Northern blot analysis detected an approximately 5 kb mRNA transcript.
Conclusions:
- The characterization of the mouse Ldlr gene provides a valuable tool for genetic studies.
- This research enhances the utility of mouse models for investigating cholesterol metabolism and gene targeting.
- The findings support the development of new therapeutic strategies for hyperlipidemia.