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Related Experiment Videos

Ovarian cancers express and process progastrin

W W van Solinge1, L Odum, J F Rehfeld

  • 1University Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.

Cancer Research
|April 15, 1993
PubMed
Summary
This summary is machine-generated.

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Ovarian tumors frequently express gastrin peptides, challenging the notion that gastrin synthesis is rare in these cancers. Malignant ovarian tumors show increased progastrin synthesis and processing compared to normal or benign tissues.

Area of Science:

  • Endocrinology
  • Oncology
  • Molecular Biology

Background:

  • Gastrin synthesis in ovarian tumors is rarely reported, often linked to Zollinger-Ellison syndrome.
  • This study investigates the prevalence of gastrin expression in ovarian neoplasms.

Purpose of the Study:

  • To determine if gastrin expression in ovarian tumors is indeed exceptional.
  • To analyze the expression and processing of progastrin in malignant and benign ovarian tumors and normal ovarian tissue.

Main Methods:

  • Utilized sequence-specific radioimmunoassays to quantify gastrin peptides.
  • Employed enzyme cleavage assays and gel chromatography to study peptide processing.
  • Analyzed 16 malignant ovarian tumors, 5 benign ovarian tumors, and 4 normal postmenopausal ovaries.

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Main Results:

  • Half of malignant ovarian tumors expressed significant amidated gastrins; the other half showed impaired amidation but higher progastrin and glycine-extended gastrin levels.
  • Malignant tumors exhibited increased progastrin and glycine-extended gastrin concentrations compared to benign tumors and normal ovaries.
  • Unsulated gastrin-17 was the predominant bioactive form in tumors with amidated gastrin.

Conclusions:

  • Postmenopausal ovaries and ovarian tumors express the gastrin gene at the peptide level.
  • Progastrin synthesis and processing are significantly elevated in malignant ovarian tumors.
  • Gastrin expression in ovarian neoplasms is more common than previously thought.