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Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein
Z Wang1, K A Tarawneh, S J Free
1Department of Biological Sciences, State University of New York, Buffalo 14260.
Current Genetics
|January 1, 1993
Abstract:
A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. The crp-5 mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed.