Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bioassay from two parabolas

M A Moussa1

  • 1Department of Community Medicine, Faculty of Medicine & Health Sciences, Al-Ain, United Arab Emirates.

Computers in Biology and Medicine
|January 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histopathological effects of azithromycin on broilers: immune system alterations and apoptotic changes.

British poultry science·2025
Same author

Prognostic significance of microRNA 17-92 cluster expression in Egyptian chronic lymphocytic leukemia patients.

Journal of the Egyptian National Cancer Institute·2021
Same author

Rational design and electrical study of conducting bionanocomposites hydrogel based on chitosan and silver nanoparticles.

International journal of biological macromolecules·2019
Same author

Clustering of trisomy 18 in Kuwait: Genetic predisposition or environmental?

Annals of Saudi medicine·2007
Same author

Plasma leptin concentration in patients with Type 2 diabetes: relationship to cardiovascular disease risk factors and insulin resistance.

Diabetic medicine : a journal of the British Diabetic Association·2005
Same author

Associations of plasma homocysteine concentration in subjects with type 2 diabetes mellitus.

Acta diabetologica·2002
Same journal

A computational model of chemically- and mechanically-induced thrombus formation in cerebral aneurysms.

Computers in biology and medicine·2026
Same journal

An improved catch fish optimization based deep learning model for Parkinson disease classification using EEG signal.

Computers in biology and medicine·2026
Same journal

Assessing the robustness of evaluation metrics for synthetic ECG signal quality.

Computers in biology and medicine·2026
Same journal

Integrating stemness and epithelial-mesenchymal transition signatures with machine learning identifies RUNX1 as a therapeutic vulnerability in colorectal cancer.

Computers in biology and medicine·2026
Same journal

Differential regional textural attributes of tongue in normal and acidity patients in the light of traditional Chinese medicine.

Computers in biology and medicine·2026
Same journal

SC-MSDNet: Spatial-consistent multi-view self-distillation for retinal OCT classification.

Computers in biology and medicine·2026
See all related articles

This study introduces a new method for estimating potency ratios in parallel curve dilution assays when the dose-response relationship is parabolic. The approach validates the quadratic model and calculates relative potency, standard deviation, and fiducial limits for unknown preparations.

Area of Science:

  • Biostatistics
  • Pharmacometrics
  • Analytical Chemistry

Background:

  • Accurate potency estimation is crucial for drug development and quality control.
  • Traditional methods often assume linear dose-response relationships, which may not always hold true.
  • Non-linear dose-response relationships, such as parabolic ones, require specialized analytical approaches.

Purpose of the Study:

  • To develop and validate a method for estimating potency ratios in parallel curve analytic dilution assays.
  • To address situations where the log dose-response relationship can be modeled by a parabola.
  • To provide tools for assessing the validity of the quadratic model and estimating relative potency.

Main Methods:

  • Utilizing analysis of variance (ANOVA) to test the adequacy and validity of the quadratic dose-response model.

Related Experiment Videos

  • Estimating the relative potency of an unknown preparation compared to a standard.
  • Calculating the standard deviation and fiducial limits for the estimated relative potency.

    • Main Results:

    • A validated method for potency ratio estimation using a quadratic model in parallel curve assays.
    • The method allows for the assessment of model fit and provides confidence intervals for potency estimates.
    • The approach is applicable to various experimental designs, including randomized blocks and completely randomized designs.

    Conclusions:

    • The proposed method offers a robust approach for potency ratio estimation when dose-response curves are parabolic.
    • This extends the applicability of analytical dilution assays to more complex biological responses.
    • The methodology can be further generalized to higher-order polynomial models for enhanced flexibility.