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Lipid hydroperoxides potentiate mesenteric artery vasoconstrictor responses

C A Hubel1, S T Davidge, M K McLaughlin

  • 1Department of Physiology, University of Cincinnati College of Medicine, OH 45267.

Free Radical Biology & Medicine
|April 1, 1993
PubMed
Summary

Lipid hydroperoxides, implicated in lipid peroxidation, alter blood vessel function by directly affecting smooth muscle. These findings suggest a role for the endothelium in regulating vascular responses to these compounds.

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Area of Science:

  • Cardiovascular Physiology
  • Oxidative Stress Biology

Background:

  • Lipid peroxidation is linked to vascular dysfunction.
  • The specific effects of hydroperoxides on vasomotor activity require further investigation.

Purpose of the Study:

  • To investigate the impact of lipid and organic hydroperoxides on the vasomotor activity of isolated rat superior mesenteric arteries.
  • To elucidate the mechanisms underlying hydroperoxide-induced changes in vascular function.

Main Methods:

  • Isolated rat superior mesenteric arteries were used to assess vasomotor responses.
  • Contractile responses to potassium and phenylephrine were measured in the presence of various hydroperoxides and enzyme treatments.
  • The role of lipid free radicals was examined using antioxidants like vitamin E and nitroblue tetrazolium.

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Main Results:

  • 13-(s)-hydroperoxylinoleic acid potentiated potassium-induced contractions.
  • Lipoxygenase-treated fatty acids, tert-butyl hydroperoxide, and hydrogen peroxide augmented agonist-induced contractions.
  • Endothelial cell denudation enhanced responses to 13-(s)-hydroperoxylinoleic acid, indicating a direct smooth muscle effect.
  • Lipid free radicals appear to mediate contractile responses, as evidenced by blunting with vitamin E and nitroblue tetrazolium.

Conclusions:

  • Lipid hydroperoxides can alter vasomotor activity, primarily through direct effects on vascular smooth muscle.
  • The vascular endothelium may modulate responses to lipid hydroperoxides.
  • These findings support a connection between lipid peroxidation and altered vascular function.