Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple and reliable method for correlative light and electron microscopic studies

J DeFelipe1, A Fairén

  • 1Instituto Cajal, Madrid, Spain.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|May 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphorylated Tau at T181 accumulates in the serum of hibernating Syrian hamsters and rapidly disappears after arousal.

Scientific reports·2024
Same author

3D Analysis of the Synaptic Organization in the Entorhinal Cortex in Alzheimer's Disease.

eNeuro·2021
Same author

3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration.

Cerebral cortex (New York, N.Y. : 1991)·2020
Same author

3D Ultrastructural Study of Synapses in the Human Entorhinal Cortex.

Cerebral cortex (New York, N.Y. : 1991)·2020
Same author

Size, Shape, and Distribution of Multivesicular Bodies in the Juvenile Rat Somatosensory Cortex: A 3D Electron Microscopy Study.

Cerebral cortex (New York, N.Y. : 1991)·2019
Same author

3D Electron Microscopy Study of Synaptic Organization of the Normal Human Transentorhinal Cortex and Its Possible Alterations in Alzheimer's Disease.

eNeuro·2019

This study introduces a simple flat-embedding method for correlative light and electron microscopy. It enables accurate ultrastructural analysis of identified neurons and their processes, crucial for understanding neural connections.

Area of Science:

  • Neuroscience
  • Microscopy techniques

Background:

  • Correlative light and electron microscopy (CLEM) is vital for high-resolution biological imaging.
  • Precisely localizing specific cellular structures across microscopy modalities remains a challenge.

Purpose of the Study:

  • To present a straightforward flat-embedding technique for CLEM.
  • To facilitate detailed ultrastructural and synaptological analysis of identified neural elements.

Main Methods:

  • A novel flat-embedding protocol for electron microscopy preparation.
  • Integration of Golgi impregnation with light and electron microscopy.
  • Serial sectioning and multi-modal imaging of neural structures.

Main Results:

  • Demonstrated accurate delineation of complex axonal arborizations in mouse cerebral cortex.

Related Experiment Videos

  • Successfully localized Golgi-impregnated neurons and processes through successive microscopy steps.
  • Validated the method for ultrastructural characterization of immunocytochemically or morphologically identified cells.

    • Conclusions:

    • The described flat-embedding method offers a robust approach for CLEM.
    • This technique enhances the systematic and accurate localization of neural structures for detailed ultrastructural studies.