Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Colorimetric chemosensitivity testing using sulforhodamine B

T Kubota1, T Takahara, M Nagata

  • 1Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.

Journal of Surgical Oncology
|February 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term results of conventional varus half-wedge proximal femoral osteotomy for the treatment of osteonecrosis of the femoral head.

The Journal of bone and joint surgery. British volume·2012
Same author

The Chiari pelvic osteotomy for patients with dysplastic hips and poor joint congruency: long-term follow-up.

The Journal of bone and joint surgery. British volume·2011
Same author

Health-related quality of life in patients with systemic lupus erythematosus after medium to long-term follow-up of hip arthroplasty.

Lupus·2007
Same author

Three-dimensional computer-aided design based design sensitivity analysis and shape optimization of the stem using adaptive p-method.

Journal of biomechanics·2005
Same author

Acquisition of multidrug resistance in recurrent breast cancer demonstrated by the histoculture drug response assay.

Anticancer research·2002
Same author

Resistant mechanisms of anthracyclines--pirarubicin might partly break through the P-glycoprotein-mediated drug-resistance of human breast cancer tissues.

Breast cancer (Tokyo, Japan)·2002

The sulforhodamine B (SRB) assay effectively measures cancer cell sensitivity to chemotherapy by assessing protein synthesis. While highly reproducible for cell lines, its predictive accuracy for human tumors in vivo is limited.

Area of Science:

  • Cellular and Molecular Oncology
  • Drug Discovery and Development

Background:

  • Accurate chemosensitivity testing is crucial for personalized cancer therapy.
  • Existing assays require optimization for sensitivity and predictive value.

Purpose of the Study:

  • To evaluate the sulforhodamine B (SRB) colorimetric assay as a method for determining cancer cell chemosensitivity.
  • To establish optimal conditions and assess the assay's sensitivity and reproducibility across different cancer models.

Main Methods:

  • Utilized sulforhodamine B (SRB) staining of cellular proteins as an endpoint marker.
  • Tested the assay on four cultured cell lines, nine human tumor xenografts, and 14 fresh surgical specimens.
  • Determined optimal SRB assay conditions: cell density, SRB concentration, and incubation time.

Related Experiment Videos

Main Results:

  • The SRB assay demonstrated high sensitivity and reproducibility for cultured cell lines, showing an 'all or none' drug effect.
  • Optimal conditions included 3-5 x 10(4) cells/well, 4% SRB, and >10 minutes incubation.
  • The predictive rate for in vivo human tumor xenografts was 63.9%.

Conclusions:

  • The SRB assay is a sensitive and reproducible method for evaluating chemosensitivity in cultured cancer cells.
  • It directly measures cellular protein synthesis, a key indicator of cell renewal and drug response.
  • The assay's predictive value for in vivo tumor chemosensitivity requires further validation.