Nicotinic acetylcholine receptor in dissociated rat nucleus tractus solitarii neurons

The ACh-activated response of the acutely dissociated neuron from the rat nucleus tractus solitarii (NTS) was investigated using conventional and perforated-patch techniques. In the present preparation, ACh and nicotine evoked inward transient currents in approximately 30% of NTS neurons tested. The ACh-activated inward current reversed the direction near 0 mV and was inhibited by D-tubocurarine in a dose-dependent manner. In contrast, muscarine resulted in no detectable changes in the NTS neurons. Some populations of the NTS had nicotinic but no muscarinic acetylcholine receptors.