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Purification of UDP-glucose dehydrogenase by hydrophobic and affinity chromatography
Abstract:
UDP-glucose dehydrogenase was purified from an extract of calf liver acetone powder by sequential chromatography on norleucine-agarose, Type 2, agarose-hexane-AMP, and UDP-hexanolamine-Sepharose. The overall yield of the highly purified enzyme was 80% and the subunit molecular weight was 52,000.
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