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High resolution polyacrylamide gel electrophoresis of Physarum polycephalum histones: improved resolution of variants
P Albert1, I Lacorre-Arescaldino, B Toublan
1Laboratoire de Biologie Cellulaire, Université de Reims Champagne-Ardenne, France.
Analytical Biochemistry
|March 1, 1993
Abstract:
An electrophoresis system providing a good resolution of all basic proteins and their variants or modified forms extracted from Physarum polycephalum nuclei was developed. This two-dimensional polyacrylamide gel electrophoresis system combines the use of an AUT gel containing a preformed gradient of urea in the first dimension and a SDS polyacrylamide gel in the second one.