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Repeated sperm injection under the zona following initial fertilization failure
S R Wiker1, M J Tucker, G Wright
1Reproductive Biology Associates, Atlanta, GA 30342.
Human Reproduction (Oxford, England)
|March 1, 1993
Summary
Repeating under-zona insemination with more sperm can lead to successful fertilization and pregnancy when initial attempts fail. This technique resulted in viable pregnancies in over 22% of human in-vitro fertilization cycles studied.
Area of Science:
- Reproductive medicine
- Embryology
- In-vitro fertilization
Background:
- Fertilization failure can occur even with micromanipulative techniques like under-zona insemination.
- Assisted reproductive technologies aim to overcome infertility challenges.
Purpose of the Study:
- To evaluate the efficacy of repeating under-zona insemination in human in-vitro fertilization (IVF) cycles following initial fertilization failure.
- To determine pregnancy and embryonic development rates after repeat insemination.
Main Methods:
- Conducted 18 human IVF cycles where under-zona insemination was repeated due to initial fertilization failure.
- Spermatozoa were added during the repeat procedure.
- Embryos were transferred based on fertilization timing (late or mixed).
Main Results:
- Two viable pregnancies (22.2% per transfer) were achieved from cycles with only late-fertilized embryos.
- Two viable pregnancies (33.3% per transfer) resulted from cycles with a mix of late- and timely-fertilized embryos.
- Three cycles (16.7%) failed to achieve fertilization even after repeat under-zona insemination.
Conclusions:
- Repeating under-zona insemination is a viable strategy to achieve fertilization, embryonic development, and pregnancy after initial failure in IVF.
- The success rate of this repeat insemination approach is significant, offering hope for patients experiencing fertilization issues.
- Further research may optimize protocols for repeat insemination to improve success rates.