Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of a decrease in muscarinic m2 mRNA in cerebellar granule cells by carbachol

P Longone1, I Mocchetti, M A Riva

  • 1Fidia Georgetown Institute for the Neurosciences, Georgetown University, School of Medicine, Washington, DC.

The Journal of Pharmacology and Experimental Therapeutics
|April 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding, predicting, and treating depression in pregnancy to improve mothers' and offspring's mental health outcomes: The HappyMums study.

Brain, behavior, & immunity - health·2025
Same author

Lattice-gas model of methane and carbon dioxide sI clathrate hydrates: A comprehensive study using analytical cluster approximation and Monte Carlo simulations.

Physical review. E·2025
Same author

Fostering inclusion in medical training: accommodations for hearing loss.

Occupational medicine (Oxford, England)·2024
Same author

Noise-induced hearing loss in the pre-industrial era: early contributions in <i>De Morbis Artificum</i> by Bernardino Ramazzini (1633-1714).

The Journal of laryngology and otology·2023
Same author

Monkeypox vaccine-related stigma.

Public health in practice (Oxford, England)·2022
Same author

Exposure to chronic stress impairs the ability to cope with an acute challenge: Modulation by lurasidone treatment.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology·2022

Carbachol treatment transiently decreases muscarinic m2 receptor mRNA in cerebellar granule cells. This suggests carbachol reduces m2 receptor gene transcription, not mRNA degradation.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Cerebellar granule cells express muscarinic m2 and m3 receptors.
  • Muscarinic acetylcholine receptors play key roles in neuronal function.

Purpose of the Study:

  • To investigate the effect of carbachol on muscarinic m2 receptor mRNA levels in cerebellar granule cells.
  • To determine the mechanism underlying carbachol-induced changes in m2 receptor mRNA.

Main Methods:

  • Treatment of cerebellar granule cells with carbachol (100 microM) for various durations.
  • Quantification of m2 receptor mRNA using ribonuclease protection assay.
  • Assessment of receptor activation specificity using methoctramine (1 microM).
  • Measurement of mRNA half-life in the presence of actinomycin D.

Related Experiment Videos

Main Results:

  • Carbachol treatment caused a transient decrease in m2 receptor mRNA levels, peaking at 6-9 hours.
  • Methoctramine selectively blocked the effect of carbachol, indicating homologous receptor activation.
  • mRNA half-life remained unchanged, suggesting transcription, not degradation, is affected.

Conclusions:

  • Carbachol treatment leads to a homologous activation of muscarinic m2 receptors.
  • The decrease in m2 receptor mRNA is likely due to reduced gene transcription.
  • These findings provide insights into the regulation of muscarinic receptor expression in the cerebellum.