Related Experiment Videos
[Heat inactivation kinetics of solubilized and immobilized immunoglobulins G]
The kinetics of thermoinactivation of rabbit antibodies against cortisol (Anti-COR, 60-82 degrees), sheep antibodies against rabbit immunoglobulins (AB, 65-77 degrees) in water solution and of AB covalently linked to activated polystyrene beads (PSB-AB, 60-77 degrees) was studied by indirect and direct ELISA methods using peroxidase-cortisol or peroxidase-rabbit immunoglobulin conjugates to detect immunocomplexes for PSB-AB. High stability of ABs and their stabilization after immobilization on PSB are shown. The Eact values and thermodynamic activation parameters of immunoglobulins thermolysis delta H*, delta S* and delta G* were calculated from Arrhenius plots of the effective inactivation rate constants versus temperature and compared with the corresponding characteristics for other proteins.
The kinetics of thermoinactivation of rabbit antibodies against cortisol (Anti-COR, 60-82 degrees), sheep antibodies against rabbit immunoglobulins (AB, 65-77 degrees) in water solution and of AB covalently linked to activated polystyrene beads (PSB-AB, 60-77 degrees) was studied by indirect and direct ELISA methods using peroxidase-cortisol or peroxidase-rabbit immunoglobulin conjugates to detect immunocomplexes for PSB-AB. High stability of ABs and their stabilization after immobilization on PSB are shown. The Eact values and thermodynamic activation parameters of immunoglobulins thermolysis delta H*, delta S* and delta G* were calculated from Arrhenius plots of the effective inactivation rate constants versus temperature and compared with the corresponding characteristics for other proteins.