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Laser-light induced chromosome aberrations in Chinese hamster cells
P Virsik-Peuckert1, G Hillrichs, W Neu
1Institute of Medical Physics and Biophysics, University of Göttingen, Germany.
Photochemistry and Photobiology
|March 1, 1993
Abstract:
Wild-type Chinese hamster cells CHO K1 and their radiosensitive mutant xrs5 were irradiated at 308 nm, using light pulses of a XeCl excimer laser with total energy fluences of 0.1 kJ/m2 to 4.08 kJ/m2. Chromosome-type and chromatid-type chromosome aberrations have been observed at pulse irradiances of 2.5 x 10(7) W/m2 and 1.7 x 10(8) W/m2, indicating that in mammalian cells DNA double-strand breaks occur already in this irradiance range. The results obtained with laser irradiation are compared with X-ray irradiation.