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Related Experiment Videos

Surface properties of RGD-peptide grafted polyurethane block copolymers: variable take-off angle and cold-stage ESCA

H B Lin1, K B Lewis, D Leach-Scampavia

  • 1Department of Chemical Engineering, University of Wisconsin-Madison 53706.

Journal of Biomaterials Science. Polymer Edition
|January 1, 1993
PubMed
Summary

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Surface analysis of peptide-grafted polyurethanes reveals that hydration enriches hard segments at the surface, enhancing endothelial cell adhesion. Dehydration causes hydrophobic soft segments to migrate back to the surface.

Area of Science:

  • Polymer Science
  • Surface Chemistry
  • Biomaterials Science

Background:

  • Polyurethane block copolymers are widely used in biomedical applications.
  • Surface properties significantly influence the biocompatibility and performance of these materials.
  • Understanding surface composition changes upon hydration is crucial for predicting in vivo behavior.

Purpose of the Study:

  • To investigate the surface composition of various polyurethane block copolymers using ESCA (Electron Spectroscopy for Chemical Analysis).
  • To correlate surface changes with hydration state and peptide grafting.
  • To evaluate the impact of surface modifications on endothelial cell adhesion.

Main Methods:

  • Variable take-off angle and cold-stage ESCA measurements were employed.

Related Experiment Videos

  • Five different polyurethane copolymers were analyzed: a PTMO-polyurethane control, a carboxylated version, and three peptide-grafted variants (GRGESY, GRGDSY, GRGDVY).
  • In vitro endothelial cell adhesion assays were performed on prehydrated samples.

    • Main Results:

    • Nitrogen signal (indicating urethane hard segments) decreased with increasing take-off angle on dry samples, suggesting surface depletion of hard segments.
    • Surface nitrogen concentration increased upon hydration for all polymers, with a more pronounced increase in peptide-grafted polyurethanes.
    • Dehydration led to decreased nitrogen concentration, indicating migration of hydrophobic PTMO soft segments to the surface.
    • Prehydrated RGD-containing peptide-grafted polyurethanes showed increased endothelial cell attachment.

    Conclusions:

    • Hydration induces enrichment of hydrophilic hard segments at the surface of polyurethanes.
    • Peptide grafting enhances this surface enrichment upon hydration.
    • The increased surface peptide density on hydrated RGD-containing polyurethanes promotes endothelial cell adhesion.
    • Surface composition dynamics are critical for the biological performance of these biomaterials.