Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Which coagulation factors interfere with the one-stage prothrombin time?

I Talstad1

  • 1Medical Department B, Haukeland Hospital, University of Bergen, Norway.

Haemostasis
|January 1, 1993
PubMed
Summary

The one-stage prothrombin time (PT) test accurately measures coagulation factors VII and X, but is less reliable for factors II and IX. Factor VII significantly impacts PT results, especially in liver disease or warfarin therapy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Why is the standardization of prothrombin time a problem?

Haemostasis·2001
Same author

[Cardiac natriuretic peptides as markers of left ventricular dysfunction].

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·1997
Same author

[Do you treat your vitamin B12 patients correctly?].

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·1996
Same author

[Homocysteine and methylmalonic acid. New tests--for what benefit?].

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·1996
Same author

Prothrombin time standardization by correction of the Pivka inhibitor.

Haemostasis·1996
Same author

[Do you treat your vitamin B 12 patients correctly?].

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·1996

Area of Science:

  • Hematology
  • Clinical Chemistry
  • Coagulation Science

Background:

  • The one-stage prothrombin time (PT) is a critical laboratory test for assessing the extrinsic pathway of coagulation.
  • Understanding how individual coagulation factors influence PT results is essential for accurate interpretation, particularly in disease states.
  • Warfarin therapy and liver disease are known to affect multiple coagulation factors, complicating PT analysis.

Purpose of the Study:

  • To investigate the specific responses of the one-stage prothrombin time (PT) to varying activities of key coagulation factors.
  • To determine the influence of individual coagulation factors (II, VII, IX, X) on PT measurements.
  • To evaluate the impact of multiple coagulation factor deficiencies on PT, particularly in the context of warfarin therapy and liver disease.

Main Methods:

  • Experimental investigation of PT activity in response to defined levels of coagulation factors VII, X, IX, and II.
  • Analysis of PT in models of multiple coagulation factor deficiencies.
  • Comparison of PT results with factor VII levels in patients undergoing warfarin therapy or with liver disease.

Main Results:

  • PT demonstrated adequate activity response to coagulation factors VII and X.
  • PT showed approximately half the expected activity response to factor IX.
  • PT response was less than expected and nonlinear for factor II; PT results closely mirrored factor VII levels in multiple deficiencies, warfarin therapy, and liver disease, with factor VII being the most reduced.

Conclusions:

  • The one-stage PT assay is highly sensitive to factor VII activity, which can significantly influence results, especially at high concentrations.
  • PT is less reliable for accurately quantifying factors II and IX compared to factors VII and X.
  • Factor VII is a primary determinant of PT values in conditions with multiple coagulation factor deficiencies, such as liver disease and warfarin treatment.

Related Experiment Videos