A review of in vitro studies: low-frequency electromagnetic fields

In vitro studies of effects of low-frequency (LF) electromagnetic (EM) fields have revealed a variety of sensitive cell-physiologic end-points. Effects have been reported on (1) DNA, RNA, and protein synthesis; (2) cell proliferation; (3) cation fluxes and binding; (4) immune responses; and (5) membrane signal transduction (i.e., hormones, enzymes, and neuro-transmitters). Typically such effects occurred as a result of short-term exposure of cells to EM at frequencies of 100 Hz or less and at low field intensities. The dependency on frequency or modulation, as well as the apparent weak cellular interaction of these LF EM fields, lacks theoretic explanation. It has not been determined whether effects are induced by electric or magnetic fields. Confounding interpretation of the results are phenomena such as (1) transient or time-delayed responses; (2) modulation- and intensity-specific effects, referred to as modulation or intensity "windows;" and (3) general lack of dose- (or dose-rate) response data or EM field thresholds. Consequently, although it is well-established that LF EM fields affect biological systems in vitro, use of these data to assess human health effects is limited. This paper reviews selected published reports of LF EM fields on in vitro systems. Where possible, relevance of the findings to occupational exposures will be assessed, principally by considering the consistency of in vitro and in vivo EM exposure effects and comparison of EM field intensities that affect in vitro systems with occupational EM exposure intensities. Finally, suggestions will be made for the direction of future in vitro research of direct pertinence to potential occupational exposure problems.