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Effect of cromakalim on micturition function in rats
A B Smith1, D L Bertelsen, S T Kau
1Department of Pharmacology, ICI Americas, Wilmington, Delaware 19897-2500.
Neurourology and Urodynamics
|January 1, 1993
Summary
Cromakalim initially reduced urine output and frequency in rats, followed by a rebound increase. These effects on micturition function were temporary, potentially linked to blood pressure changes.
Area of Science:
- Pharmacology
- Urology
Background:
- Existing research on cromakalim focuses on bladder contractility.
- No studies have investigated cromakalim's impact on micturition function in conscious rats.
Purpose of the Study:
- To evaluate the effects of cromakalim on micturition function in conscious rats.
- To assess changes in urine output, frequency, voiding volume, and blood pressure.
Main Methods:
- Intravenous administration of cromakalim to saline-diuresed and non-diuresed rats.
- Monitoring of urine output, micturition frequency, voiding volume, and mean arterial pressure.
- Dose-ranging study (0.1, 0.3, 1.0 mg/kg).
Main Results:
- Cromakalim caused dose-dependent decreases in urine output and frequency in the initial 3 hours.
- A significant rebound increase in urine output and frequency was observed between 3-6 hours.
- Mean arterial pressure dropped by 50% immediately after administration, with recovery over 3 hours.
- Similar effects were noted in both diuresed and non-diuresed rats.
Conclusions:
- Cromakalim transiently alters micturition function in rats.
- The initial decrease in urine output may be secondary to cromakalim-induced hypotension.
- Further research is needed to understand the long-term implications and mechanisms.