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Updated: Aug 12, 2026

Staining Proteins in Gels
Published on: July 8, 2008
A robust, high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels
1Division of Biology, California Institute of Technology, Pasadena 91125.
Abstract:
The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects. We describe in this paper a hierarchical algorithm that provides a robust, high-sensitivity solution to this problem, which can be easily adapted to a variety of experimental situations. The software implementation of this algorithm functions as part of a complete package designed for general protein gel analysis applications.
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