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Comparative study of biochemical parameters and kinetic properties of dopamine-beta-hydroxylase activity from cat and
D Fortin1, J F Coulon, A G Roberge
1INRS-Santé, Université du Québec, Pointe-Claire, Canada.
Abstract:
The specific adrenal dopamine-beta-hydroxylase activity measured in cat and rat was: (1) two times higher in cat than in rat; (2) significantly enhanced by ascorbate (up to 5 mM) that acts as a major activator; (3) differently affected by exogenous copper added to the incubation medium, leading to a decrease with large concentrations; and (4) similar, according to the kinetic parameters, thereby demonstrating a greater affinity for ascorbate than for tyramine.