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[Characterization of Acholeplasma Strains by Horizontal Polyacrylamide Flat Gel Electrophoresis (author's transl)]
Abstract:
Proteins extracted with phenol-acetic acid-water (2:1:0.5, w/v/v) from Acholeplasma laidlawii (PG 8), A. granularum (BTS-39), A. oculi (19L), A. modicum (PG 49), A. axanthum (S743) and the Acholeplasma strains C1 and C112 (which were isolated from aborted horse foetuses) were compared by electrophoresis in horizontal acidic polyacylamide flat gel using the electrophoresis equipment LKB Multiphor 2117. In this system the gels are not prepared in the electrophoresis chamber but between glas plates. For electrophoresis they are applied onto a special cooling plate. This makes it possible to produce a number of identical gels (from the same gel mixture and polymerized under the same conditions) what can be important for comparing investigations. The gels can be stored for more than 4 weeks in the refrigerator at +4 degrees C. Marked differences were observed between the electrophoretic patterns of each of the established species and the horse strains C1 and C112. The results are in agreement with those obtained in serological investigations in which the strains C1 and C112 were different from the established Acholeplasma species.
Insights
Electrophoresis of Acholeplasma proteins revealed distinct patterns between established species and two horse strains. These findings align with serological data, differentiating the horse isolates.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Context:
- Acholeplasma species are important in veterinary and comparative medicine.
- Distinguishing between Acholeplasma strains is crucial for accurate diagnosis and epidemiological studies.
- Previous serological investigations suggested differences in horse-derived strains.
Purpose:
- To compare the protein profiles of established Acholeplasma species with strains isolated from aborted horse foetuses using acidic polyacrylamide gel electrophoresis.
- To evaluate the utility of electrophoretic protein patterns as a taxonomic tool for Acholeplasma.
- To correlate electrophoretic findings with existing serological data.
Summary:
- Proteins were extracted from seven Acholeplasma strains (A. laidlawii, A. granularum, A. oculi, A. modicum, A. axanthum, and two horse isolates C1 and C112) using a phenol-acetic acid-water method.
- Electrophoresis was performed on horizontal acidic polyacrylamide flat gels using LKB Multiphor 2117 equipment, allowing for the production and storage of identical gels.
- Distinct electrophoretic protein patterns were observed for each established Acholeplasma species and the two horse strains, indicating significant genetic and/or phenotypic divergence.
Impact:
- The study provides a robust electrophoretic method for differentiating Acholeplasma strains, complementing serological techniques.
- The observed protein pattern differences support the classification of horse strains C1 and C112 as distinct from established Acholeplasma species.
- This research contributes to the accurate identification and characterization of Mycoplasma-related organisms in animal health contexts.