A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids
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M J Keherly1, J Papaconstantinou, J L McCombs
1Department of Human Biological Chemistry and Genetics, University of Texas, Medical Branch, Galveston 77555-0359.
Experimental Cell Research
|April 1, 1993
Abstract:
We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. This procedure not only provided a simple distinction between human and mouse chromosomes, but it also allowed the visualization and monitoring of human sequences present in interspecific translocations and subchromosomal fragments.