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Related Experiment Videos

Inhaled FMLP increases microvascular permeability in the rabbit trachea

S C Bell1, A C Rynell, M J Matheson

  • 1Department of Thoracic Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|March 1, 1993
PubMed
Summary

Inhaled N-formyl-methionyl-leucyl-phenylalanine (FMLP) significantly increases microvascular permeability in rabbit trachea. This effect is modulated by cholinergic blockade and neutral endopeptidase (NEP) inhibition, suggesting a role for endogenous opioids.

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Area of Science:

  • Pulmonary Pharmacology
  • Vascular Biology
  • Immunopharmacology

Background:

  • N-formyl-methionyl-leucyl-phenylalanine (FMLP) is a potent chemoattractant peptide.
  • FMLP's role in modulating airway microvascular permeability is not fully understood.
  • Understanding FMLP's effects is crucial for respiratory disease research.

Purpose of the Study:

  • To investigate the impact of inhaled FMLP on rabbit tracheal microvascular permeability.
  • To explore the modulatory effects of cholinergic antagonism, neutral endopeptidase (NEP) inhibition, and opioid receptor modulation on FMLP-induced permeability.
  • To elucidate the mechanisms underlying FMLP's effects on airway vasculature.

Main Methods:

  • New Zealand White rabbits were used, with intravenous administration of control solutions or specific inhibitors/antagonists.

Related Experiment Videos

  • Evans blue dye was administered intravenously, followed by nebulized FMLP or control.
  • Tracheal microvascular permeability was quantified by measuring extravascular Evans blue concentration spectrophotometrically.

    • Main Results:

    • FMLP significantly increased tracheal microvascular permeability compared to controls.
    • Cholinergic blockade and NEP inhibition (thiorphan, phosphoramidon) significantly reduced the FMLP-induced increase in permeability.
    • The inhibitory effect of NEP inhibition was reversed by the opioid antagonist naloxone, while morphine had no significant effect.

    Conclusions:

    • Inhaled FMLP increases microvascular permeability in the rabbit trachea, potentially contributing to airway resistance changes.
    • NEP inhibition attenuates FMLP's effect, possibly via modulation of endogenous opioid pathways.
    • The FMLP-induced increase in microvascular permeability is partially mediated by vagal pathways.