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Related Experiment Videos

H-ras-1 point mutations in soft tissue sarcomas

W Wilke1, M Maillet, R Robinson

  • 1Department of Pathology, University of Iowa, Iowa City.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
|March 1, 1993
PubMed
Summary

RAS mutations, specifically in H-ras-1, were investigated in human sarcomas. Three cases of malignant fibrous histiocytoma and embryonal rhabdomyosarcoma showed specific codon 12 mutations, suggesting relevance in sarcoma development.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The H-ras-1 protooncogene is frequently activated by point mutations in human cancers.
  • Activating mutations in codons 12, 13, or 61 of H-ras-1 are common in various tumor types.
  • The presence of ras mutations in human sarcomas remains to be fully elucidated.

Purpose of the Study:

  • To investigate the occurrence and types of H-ras-1 mutations in human soft tissue sarcomas.
  • To determine if specific H-ras-1 mutations are associated with particular sarcoma subtypes.

Main Methods:

  • DNA extraction from six malignant fibrous histiocytomas (MFH), four rhabdomyosarcomas (ER), and one leiomyosarcoma.
  • Polymerase chain reaction (PCR) amplification of H-ras-1 regions flanking codons 12/13 and 61.

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  • Automated DNA sequencing of amplified regions and comparison with normal and known mutated DNA controls.

    • Main Results:

    • Three sarcomas exhibited H-ras-1 mutations, all located in codon 12.
    • One embryonal rhabdomyosarcoma had a G-to-T transversion at codon 12 (Glycine to Valine).
    • Two malignant fibrous histiocytomas showed G-to-A transitions at codon 12 (Glycine to Aspartic Acid).

    Conclusions:

    • H-ras-1 mutations at codon 12 are present in a subset of malignant fibrous histiocytomas and embryonal rhabdomyosarcomas.
    • These findings suggest a potential role for H-ras-1 mutations in the oncogenesis of these specific sarcoma types.
    • Further research involving larger cohorts and analysis of N-ras and K-ras mutations is warranted to confirm the significance of ras mutations in human soft tissue sarcomas.