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Related Experiment Videos

Aggregated human immunoglobulins bind to modified proteins

J P López-Bote1, C Langa, P Lastres

  • 1Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.

Scandinavian Journal of Immunology
|May 1, 1993
PubMed
Summary

Aggregated human immunoglobulins (IgG) bind to modified proteins, not native ones. These IgG aggregates are found in rheumatoid arthritis patients and may play a role in oxidized protein metabolism.

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Area of Science:

  • Immunology
  • Biochemistry
  • Rheumatology

Background:

  • Human immunoglobulins (IgG) can interact with modified proteins.
  • Aggregated IgG forms high molecular weight complexes.
  • These complexes are found in vivo in rheumatoid arthritis patients.

Purpose of the Study:

  • To investigate the interaction between human immunoglobulins and modified proteins.
  • To determine the in vivo relevance of IgG aggregates.
  • To explore the role of aggregated IgG in oxidized protein metabolism.

Main Methods:

  • In vitro incubation of human immunoglobulins at elevated temperatures.
  • Gel filtration chromatography to analyze IgG complexes.
  • Detection of IgG aggregates in synovial fluids from rheumatoid arthritis patients.

Related Experiment Videos

  • Competition assays with modified proteins and aggregated IgG.
  • Assessment of aggregated IgG's effect on foam cell formation.

    • Main Results:

    • Human immunoglobulins treated at 55°C bind to maleylated bovine serum albumin (mBSA) but not unmodified BSA.
    • mBSA binding is linked to high molecular weight aggregated IgG.
    • Aggregated IgG can be generated in vitro and detected in rheumatoid arthritis synovial fluids.
    • Aggregated IgG binds to other modified proteins like maleylated human serum albumin (mHSA) and acetylated low-density lipoprotein (Ac-LDL).
    • Aggregated IgG enhances Ac-LDL-dependent foam cell formation.

    Conclusions:

    • Aggregated IgG exhibits binding capacity for modified proteins.
    • These IgG aggregates are present in vivo in rheumatoid arthritis.
    • Aggregated IgG may be involved in the metabolism of oxidized proteins.