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Related Experiment Videos

Apolipoprotein E: binding to soluble Alzheimer's beta-amyloid

T Wisniewski1, A Golabek, E Matsubara

  • 1Department of Pathology, NY Univ Medical Center, NY 10016.

Biochemical and Biophysical Research Communications
|April 30, 1993
PubMed
Summary

Apolipoprotein E (apo E) binds to amyloid beta (A beta) peptides in cerebrospinal fluid. This interaction may be crucial for the formation of amyloid plaques, a hallmark of Alzheimer's disease.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Apolipoprotein E (apo E) is found in senile plaques associated with Alzheimer's disease.
  • Amyloid beta (A beta) is a soluble peptide present in cerebrospinal fluid (CSF) and other biological fluids.

Purpose of the Study:

  • To investigate the binding of apo E and other amyloid-associated proteins to A beta peptides in CSF.
  • To determine if apo E interacts with soluble A beta (sA beta).

Main Methods:

  • Synthetic A beta peptides immobilized on affinity membranes were incubated with CSF.
  • Enzyme-linked immunosorbent assay (ELISA) and ligand binding studies were employed.

Main Results:

  • Apo E and apolipoprotein J (Apo J) bound to the A beta-coated membranes.

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  • High avidity binding was observed between A beta peptides and apo E.

    • Conclusions:

    • Apo E and potentially other related proteins can bind to both amyloidogenic and soluble forms of A beta.
    • This interaction is suggested to play a critical role in amyloid formation and Alzheimer's pathogenesis.