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Platelet-activating factor stimulates protein kinase C translocation in cerebral microvessels
R E Catalán1, A M Martínez, M D Aragonés
1Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Biochemical and Biophysical Research Communications
|April 30, 1993
Abstract:
The effects of platelet-activating factor (PAF) on distribution of protein kinase C activity in brain microvessels have been examined. Our results indicate that PAF caused an increase of protein kinase C activity in membrane, accompanied by a loss of activity in the cytosol. This effect resulted to be dose-dependent and the translocation was evident after 30 min of PAF treatment. These results suggest that PAF could play a role in transport processes in the blood-brain barrier, involving protein phosphorylation by activation of protein kinase C.